wheat:gateway+genes

4x: GPC-A1 MT: GPC-B2 WT

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Given name + traits Accession # + images 
+ dates
Genetic data OriginNOTE + collected Ancestry Other names + other # Notes
4x: GPC-A1 MT: GPC-B2 WT
Triticum durum
status = Genetic stock
order = available
# PI 673414

@ USDA-ARS (USA) INFO 

received: 28/11/2014

United States, California (developed)
see narrative
GPC1 and GPC2 are paralogous NAC-domain transcription factors expressed in flag leaves after anthesis which have putative roles in senescence and micronutrient translocation to the grain. An EMS (Ethyl Methane Sulfonate)-treated (1%) population of the tetraploid wheat (Triticum turgidum L. subsp. durum (Desf.) var. Kronos) was screened for mutations in the GPC-A1 and GPC-B2 genes. GPC-B1 carries a frame-shift mutation resulting in a non-functional protein in this variety. Knockout mutations were identified in M3 lines (GPC-A1 W114*, GPC-B2 = W109*), backcrossed twice to wild-type non-mutangenized Kronos and combined to generate BC2F2 lines segregating for these mutant alleles. WT=wild-type; MT-mutant-type. See PI 673413 and 673415 for the other mutant combinations.
Given name + traits Accession # + images 
+ dates
Genetic data OriginNOTE + collected Ancestry Other names + other # Notes
4x: GPC-A1 MT: GPC-B2 WT
Triticum durum
status = Genetic stock
order = available
# PI 673414

@ USDA-ARS (USA) INFO 

received: 28/11/2014

United States, California (developed)
see narrative
GPC1 and GPC2 are paralogous NAC-domain transcription factors expressed in flag leaves after anthesis which have putative roles in senescence and micronutrient translocation to the grain. An EMS (Ethyl Methane Sulfonate)-treated (1%) population of the tetraploid wheat (Triticum turgidum L. subsp. durum (Desf.) var. Kronos) was screened for mutations in the GPC-A1 and GPC-B2 genes. GPC-B1 carries a frame-shift mutation resulting in a non-functional protein in this variety. Knockout mutations were identified in M3 lines (GPC-A1 W114*, GPC-B2 = W109*), backcrossed twice to wild-type non-mutangenized Kronos and combined to generate BC2F2 lines segregating for these mutant alleles. WT=wild-type; MT-mutant-type. See PI 673413 and 673415 for the other mutant combinations.

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